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Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma

AUTHORS

Solmaz Khaghani Razi Abad 1 , Mehdi Pooladi 1 , Mehrdad Hashemi 2 , Afshin Moradi 3 , Sara Sobhi 4 , Alireza Zali 5 , Masoumea Mousavi 6 , Hakimeh Zali 6 , Mona Zamanian Azodi 6 , Azadeh Rakhshan 7 , Reza Vafaee 6 , Mostafa Rezaei Tavirani 6 , *

1 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Dept. of Neurosurgery, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7 Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Khaghani Razi Abad S , Pooladi M, Hashemi M, Moradi A, Sobhi S, et al. Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma, Int J Cancer Manag. 2013 ; 6(Supplement):e80454.

ARTICLE INFORMATION

International Journal of Cancer Management: 6 (Supplement); e80454
Published Online: December 30, 2013
Article Type: Research Article
Received: January 09, 2013
Accepted: February 12, 2013

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Abstract

Background: Oligodendrogliomas are sub-types of Gliomas with an enriched net of branching capillary. A vast amount of Oligodendroglial (approximately 60-90%) demonstrates a loss of 1p and 19q chromosomes, and their response to radio and chemotherapy treatments are considerably more favorable and less aggressive. Rho GDP inhibitor is a protein which controls the activity and distribution of GTPase in cells. In cancer cells with 1pLOH deletion, Rho GDP inhibitor protein can be anti-apoptotic. Here understanding of molecular diagnosis of Oligodendroglioma tumors is investigated via proteomic tools.

Methods: Proteins of tumor and normal brain tissues are extracted and separated by Two-Dimensional Gel (2DG) Electrophoresis method and the spots were then analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by pI, molecular weights and data banks.

Results: The 2D gels of normal and patient were provided and compared. As a result,high resolution analysis showed that there are totally 1328 spots in the gel. Bioinformatic analysis of the gels revealed that Rho GDI is one of the downregulated proteins.

Conclusion: It was revealed that Rho GDP inhibitor deactivates GTPProtein complex through disintegration of GTP from Rho proteins, leading to prevention of Rho GDP to return to its cycling.

Keywords

Rho GDP Oligodendroglioma Two-Dimensional electrophoresis Proteomics Brain tumors

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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