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The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines

AUTHORS

Mohsen Alipour 1 , Seyed Jalal Zargar 1 , * , Shahrokh Safarian 1 , Shamileh Fouladdel 2 , Ebrahim Azizi 3 , Naser Jafargholizadeh 1

1 Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

2 Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3 Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

How to Cite: Alipour M, Zargar S J, Safarian S, Fouladdel S, Azizi E, et al. The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines, Int J Cancer Manag. 2013 ; 6(2):e80399.

ARTICLE INFORMATION

International Journal of Cancer Management: 6 (2); e80399
Published Online: June 30, 2013
Article Type: Research Article
Received: November 20, 2012
Accepted: February 18, 2013

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Abstract

Background: The Bcl-2 protein family members have known as essential controllers of mitochondrial pathway of apoptosis. Bax is a proapoptotic member of Bcl-2 protein family, which is well known to play crucial roles for apoptosis control. bax has been implicated as potential tumor suppressor in certain solid tumors such as breast and colorectal carcinoma. DNA methylation of promoter associated CpG islands has known as a common mechanism for gene inactivation in tumor cells.

Methods: The Methylation Specific PCR (MSP) has used to find the methylation profile of the bax gene promoter CpG islands in colorectal and breast cancer cell lines.

Results: We have not detected any kind of "CpG islands hypermethylation" in promoter region of the bax gene in T47D, MCF7 (as ER positive), MDA-MB-231 and MDA-MB-468 (as ER negative) breast carcinoma-derived cell lines and colorectal cancer cell lines H29 and Caco II.

Conclusion: It seems that CpG island methylation could not play the main role in down-regulation of bax gene in breast and colon cancers.

Keywords

CpG island DNA methylations Polymerase Chain Reaction (PCR) Breast cancer Colorectal cancer

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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