International Journal of Cancer Management

Published by: Kowsar

Substrate-Dependent Activity of ERK and MEK Proteins in Breast Cancer (MCF7), and Kidney Embryonic (Hek-293) Cell Lines, Cultured on Different Substrates

Aliakbar Taherian 1 , * , Thomas A. Haas 2 and Abdoulhossein Davoodabadi 3
Authors Information
1 Kashan Gametogenesis Research Centre, Kashan University of Medical Sciences, Kashan, IR Iran
2 Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada
3 Department of Surgery, Kashan University of Medical Sciences, Kashan, IR Iran
Article information
  • Iranian Journal of Cancer Prevention: October 28, 2015, 8 (5); e3909
  • Published Online: October 27, 2015
  • Article Type: Research Article
  • Received: August 25, 2015
  • Accepted: October 4, 2015
  • DOI: 10.17795/ijcp-3909

To Cite: Taherian A, Haas T A, Davoodabadi A. Substrate-Dependent Activity of ERK and MEK Proteins in Breast Cancer (MCF7), and Kidney Embryonic (Hek-293) Cell Lines, Cultured on Different Substrates, Int J Cancer Manag. 2015 ; 8(5):e3909. doi: 10.17795/ijcp-3909.

Copyright © 2015, Iranian Journal of Cancer Prevention.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Falandry C, Canney PA, Freyer G, Dirix LY. Role of combination therapy with aromatase and cyclooxygenase-2 inhibitors in patients with metastatic breast cancer. Ann Oncol. 2009; 20(4): 615-20[DOI][PubMed]
  • 2. Baselga J, Mendelsohn J. The epidermal growth factor receptor as a target for therapy in breast carcinoma. Breast Cancer Res Treat. 1994; 29(1): 127-38[DOI]
  • 3. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015; 65(1): 5-29[DOI][PubMed]
  • 4. Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA Cancer J Clin. 2014; 64(1): 9-29[DOI][PubMed]
  • 5. Viale G. The current state of breast cancer classification. Ann Oncol. 2012; 23 Suppl 10-10[DOI][PubMed]
  • 6. Pierre FH, Santarelli RL, Allam O, Tache S, Naud N, Gueraud F, et al. Freeze-dried ham promotes azoxymethane-induced mucin-depleted foci and aberrant crypt foci in rat colon. Nutr Cancer. 2010; 62(5): 567-73[DOI][PubMed]
  • 7. van Dijk M, Goransson SA, Stromblad S. Cell to extracellular matrix interactions and their reciprocal nature in cancer. Exp Cell Res. 2013; 319(11): 1663-70[DOI][PubMed]
  • 8. Yakovlev S, Zhang L, Ugarova T, Medved L. Interaction of fibrin(ogen) with leukocyte receptor alpha M beta 2 (Mac-1): further characterization and identification of a novel binding region within the central domain of the fibrinogen gamma-module. Biochemistry. 2005; 44(2): 617-26[DOI][PubMed]
  • 9. Flick MJ, Du X, Degen JL. Fibrin (ogen)-αMβ2 interactions regulate leukocyte function and innate immunity in vivo. Exp Biol Med. 2004; 229(11): 1105-10
  • 10. Kadler KE, Hill A, Canty-Laird EG. Collagen fibrillogenesis: fibronectin, integrins, and minor collagens as organizers and nucleators. Curr Opin Cell Biol. 2008; 20(5): 495-501[DOI][PubMed]
  • 11. Magnusson MK, Mosher DF. Fibronectin : Structure, Assembly, and Cardiovascular Implications. Arterioscler Thromb Vasc Biol. 1998; 18(9): 1363-70[DOI]
  • 12. Pereira M, Rybarczyk BJ, Odrljin TM, Hocking DC, Sottile J, Simpson-Haidaris PJ. The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix. J Cell Sci. 2002; 115: 609-17[PubMed]
  • 13. Boulton TG, Nye SH, Robbins DJ, Ip NY, Radzlejewska E, Morgenbesser SD, et al. ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. Cell. 1991; 65(4): 663-75[DOI]
  • 14. Yao Y, Li W, Wu J, Germann UA, Su MS, Kuida K, et al. Extracellular signal-regulated kinase 2 is necessary for mesoderm differentiation. Proc Natl Acad Sci U S A. 2003; 100(22): 12759-64[DOI][PubMed]
  • 15. Saba-El-Leil MK, Vella FD, Vernay B, Voisin L, Chen L, Labrecque N, et al. An essential function of the mitogen-activated protein kinase Erk2 in mouse trophoblast development. EMBO Rep. 2003; 4(10): 964-8[DOI][PubMed]
  • 16. Hatano N, Mori Y, Oh-hora M, Kosugi A, Fujikawa T, Nakai N, et al. Essential role for ERK2 mitogen-activated protein kinase in placental development. Genes Cells. 2003; 8(11): 847-56[DOI]
  • 17. Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, et al. ERK1-Deficient Mice Show Normal T Cell Effector Function and Are Highly Susceptible to Experimental Autoimmune Encephalomyelitis. J Immunol. 2005; 175(4): 2374-80[DOI]
  • 18. Pagès G, Guérin S, Grall D, Bonino F, Smith A, Anjuere F, et al. Defective Thymocyte Maturation in p44 MAP Kinase (Erk 1) Knockout Mice. Science. 1999; 286(5443): 1374-7[DOI]
  • 19. Bost F, Aouadi M, Caron L, Even P, Belmonte N, Prot M, et al. The Extracellular Signal-Regulated Kinase Isoform ERK1 Is Specifically Required for In Vitro and In Vivo Adipogenesis. Diabetes. 2005; 54(2): 402-11[DOI]
  • 20. Mazzucchelli C, Vantaggiato C, Ciamei A, Fasano S, Pakhotin P, Krezel W, et al. Knockout of ERK1 MAP Kinase Enhances Synaptic Plasticity in the Striatum and Facilitates Striatal-Mediated Learning and Memory. Neuron. 2002; 34(5): 807-20[DOI]
  • 21. Vantaggiato C, Formentini I, Bondanza A, Bonini C, Naldini L, Brambilla R. ERK1 and ERK2 mitogen-activated protein kinases affect Ras-dependent cell signaling differentially. J Biol. 2006; 5(5): 14[DOI][PubMed]
  • 22. Pawson T, Nash P. Protein-protein interactions define specificity in signal transduction. Genes Dev. 2000; 14(9): 1027-47[PubMed]
  • 23. Pawson T. Protein modules and signalling networks. Nature. 1995; 373(6515): 573-80[DOI][PubMed]
  • 24. Pawson T. Signaling Through Scaffold, Anchoring, and Adaptor Proteins. Science. 1997; 278(5346): 2075-80[DOI]
  • 25. Hunter T. Protein kinases and phosphatases: The Yin and Yang of protein phosphorylation and signaling. Cell. 1995; 80(2): 225-36[DOI]
  • 26. Kleinman HK, Philp D, Hoffman MP. Role of the extracellular matrix in morphogenesis. Curr Opin Biotechnol. 2003; 14(5): 526-32[DOI]
  • 27. Hoshino R, Chatani Y, Yamori T, Tsuruo T, Oka H, Yoshida O, et al. Constitutive activation of the 41-/43-kDa mitogen-activated protein kinase signaling pathway in human tumors. Oncogene. 1999; 18(3): 813-22[DOI][PubMed]
  • 28. Kuschel C, Steuer H, Maurer A, Kanzok B, Stoop R, Angres B. Cell adhesion profiling using extracellular matrix protein microarrays. BioTech. 2006; 40(4): 523-31[DOI]
  • 29. Taherian A, Li X, Liu Y, Haas TA. Differences in integrin expression and signaling within human breast cancer cells. BMC Cancer. 2011; 11: 293[DOI][PubMed]
  • 30. Xu H, Bihan D, Chang F, Huang PH, Farndale RW, Leitinger B. Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PLoS One. 2012; 7(12)[DOI][PubMed]
  • 31. Barczyk M, Carracedo S, Gullberg D. Integrins. Cell Tissue Res. 2010; 339(1): 269-80[DOI][PubMed]
  • 32. Leitinger B. Transmembrane collagen receptors. Annu Rev Cell Dev Biol. 2011; 27: 265-90[DOI][PubMed]
  • 33. Popova SN, Lundgren-Akerlund E, Wiig H, Gullberg D. Physiology and pathology of collagen receptors. Acta Physiol (Oxf). 2007; 190(3): 179-87[DOI][PubMed]
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