Efficient Lentiviral Transduction of Adipose Tissue-Derived Mouse Mesenchymal Stem Cells and Assessment of Their Penetration in Female Mice Cervical Tumor Model

AUTHORS

Azra Kenarkoohi 1 , Masoud Soleimani 2 , * , Taravat Bamdad 1 , Hoorieh Soleimanjahi 1 , Hajar Estiri 3 , Mohammad Hadi Razavi-Nikoo 1

1 Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Dept. of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Dept. of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran

How to Cite: Kenarkoohi A, Soleimani M , Bamdad T, Soleimanjahi H, Estiri H , et al. Efficient Lentiviral Transduction of Adipose Tissue-Derived Mouse Mesenchymal Stem Cells and Assessment of Their Penetration in Female Mice Cervical Tumor Model, Int J Cancer Manag. 2014 ; 7(4):e80562.

ARTICLE INFORMATION

International Journal of Cancer Management: 7 (4); e80562
Published Online: December 31, 2014
Article Type: Research Article
Received: July 26, 2014
Accepted: October 01, 2014

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Abstract

Background: Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.

Methods: MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.

Results: Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.

Conclusion: The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

Keywords

Lentivector Adipose Tissue-Derived mouse Mesenchymal Stem Cells cervical cancer Transduction

© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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