The Effect of Interleukin 36 Gene Therapy in the Regression of Tumor

AUTHORS

Shiva Solahaye-Kahnamouii 1 , Farrokh Farhadi 2 , Mahni Rahkare-Farshi 3 , Farzaneh Pakdel 4 , Atabak Kashefimehr 5 , Firouz Pouralibaba 4 , * , Gholamreza Shirani 1 , Mohammad Bayat 1 , Abbas Karimi 1

1 Dept. of Oral Maxillofacial Surgery, Tehran University of Medical Science, Tehran, Iran

2 Dept. of Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Science, Tabriz, Iran

3 Dept. of Pediatrics, Nursing and Midwifery Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

4 Dept. of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

5 Dept. of Periodontology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

How to Cite: Solahaye-Kahnamouii S, Farhadi F, Rahkare-Farshi M, Pakdel F, Kashefimehr A, et al. The Effect of Interleukin 36 Gene Therapy in the Regression of Tumor, Int J Cancer Manag. 2014 ; 7(4):e80557.

ARTICLE INFORMATION

International Journal of Cancer Management: 7 (4); e80557
Published Online: December 31, 2014
Article Type: Research Article
Received: April 26, 2014
Accepted: September 16, 2014

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Abstract

Background: Cancer immunotherapy attempts to stimulate the immune system to reject and destroy tumors and is one of the cancer treatment strategies. Recently, interluekin36 (IL36) has been used as immunotherapeutic agents in cancer gene therapy. Present study investigated that the IL36 gene therapy effects on the regression of tumor masses in mouse model. Aim of this study is determination of the gene therapy effects by IL36 in the regression of tumor masses in mouse model.

Methods: To study the therapeutic efficacy of this cytokine, WEHI-164 tumor cells were transected with mIL36 plasmids. ELISA test was used to check cytokine production by transected cells. To establish fibro sarcoma mouse model, Tumoral transfected cells were injected subcutaneously to inoculate tumor in BALB/C mice. Tumor volumes were measured by caliper. Mice were sacrificed and tumors were extracted. The expression of IL36 and IFN-γ was studied with Real-time PCR and immunoblotting. The expression of Ki-67 (a tumor proliferation marker) in tumor masses was studied by immunohistochemistry staining. In this study we had 2 groups which are treated with IL-36 and Untreated with IL-36 as a blank.

Results: The group treated with IL36 indicated decrease of tumor mass volume (p<0.001). The results of western blotting and real-time PCR showed the IL36 expression increased in the group treated with IL36 (with relative expression of 1.9).

Conclusion: Immunohistochemistry staining indicated that the Ki67expression has been reduced in the group interfered with IL36. IL36 gene therapy has therapeutic effects on the regression of tumor masses in fibro sarcoma mouse model.

Keywords

IL-36 Gene therapy tumor Mass ELISA Immunobloting

© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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