The Study of “Dihydropyrimidinase Related Proteins (DRPs)” Expression Changes Influence in Malignant Astrocytoma Brain Tumor

AUTHORS

Mehdi Pooladi 1 , * , Mostafa Rezaei-Tavirani 2 , Mehrdad Hashemi 3 , Saeed Hesami-Tackallou 4 , Solmaz Khaghani-Razi-Abad 1 , Afshin Moradi 5 , Ali Reza Zali 6 , Masoumea Mousavi 2 , Azadeh Rakhshan 5 , Leila Firozi-dalvand 2 , Roghaye Omidi 2

1 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Dept. of Molecular Genetics, Tehran Medical Branch, Islamic Azad University Tehran, Iran 4. Dept. of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Andorra

4 Dept. of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran

5 Dept. of Pathology, Shohada Hospital, Shahid Beheshti University Of Medical Science, Tehran, Iran

6 Dept. of Neurosurgery, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Pooladi M , Rezaei-Tavirani M, Hashemi M, Hesami-Tackallou S, Khaghani-Razi-Abad S, et al. The Study of “Dihydropyrimidinase Related Proteins (DRPs)” Expression Changes Influence in Malignant Astrocytoma Brain Tumor, Int J Cancer Manag. 2014 ; 7(3):e80540.

ARTICLE INFORMATION

International Journal of Cancer Management: 7 (3); e80540
Published Online: September 30, 2014
Article Type: Research Article
Received: January 27, 2014
Accepted: March 25, 2014

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Abstract

Background: Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.

Methods: The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.

Results: The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.

Conclusion: This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity

Keywords

Asterocytoma Proteomics DRP and 2DG Electrophoresis

© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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