Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line

AUTHORS

Masumeh Gorgian Mohammadi 1 , Mehdi Hedayati 2 , * , Nosratollah Zarghami 3 , Sara Ghaemmaghami 1 , Mojtaba Mohaddes 4

1 Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran

2 Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran

3 Dept. of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

4 Dept. of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

How to Cite: Gorgian Mohammadi M, Hedayati M , Zarghami N, Ghaemmaghami S, Mohaddes M . Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line, Int J Cancer Manag. 2013 ; 6(3):e80431.

ARTICLE INFORMATION

International Journal of Cancer Management: 6 (3); e80431
Published Online: September 30, 2013
Article Type: Research Article
Received: July 16, 2013
Accepted: August 17, 2013

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line.

Methods: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion.

Results: Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed.

Conclusion: Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer.

Keywords

Visfatin Nampt Resistin Gastric cancer AGS cell line

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Fulltext

The Full text is available in PDF.

COMMENTS

LEAVE A COMMENT HERE: