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The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor

AUTHORS

Mehdi Pooladi 1 , Sara Sobhi 2 , Solmaz Khaghani Razi Abad 3 , Mehrdad Hashemi 4 , Afshin Moradi 5 , Ali Reza Zali 6 , Masoumea Mousavi 7 , Hakimeh Zali 7 , Mona Zamanian Azodi 7 , Azadeh Rakhshan 2 , Mostafa Rezaei Tavirani 7 , *

1 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences; Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehrans, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

5 Cancer Research Center, Shahid Beheshti University of Medical Sciences; Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Dept. of Neurosurgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Pooladi M, Sobhi S, Khaghani Razi Abad S, Hashemi M , Moradi A, et al. The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor, Int J Cancer Manag. 2013 ; 6(Supplement):e80445.

ARTICLE INFORMATION

International Journal of Cancer Management: 6 (Supplement); e80445
Published Online: December 30, 2013
Article Type: Research Article
Received: November 19, 2012
Accepted: January 14, 2013

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Abstract

Background: Asterocytoma is the most common primary human brain tumor which has the most lethal hazard among tumors in human Central Nervous System. Heat Shock Proteins play an active role in cancer cells pathways through their effects on tumor cell proliferation, tumor differentiation, malignancy, metastasis, and cell death. HSP70 is a 70 KD protein that its expression change has been reported in various kinds of cancers. Here a proteomic activity for molecular diagnosis of Asterocytomas tumors is designed.

Methods: Proteins of tumor and normal brain tissues were extracted and then evaluated by Bradford test. In this study, the proteins were separated by 2DG Electrophoresis method, and then the spots were analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by Isoelectric point (pI), molecular weights and data banks.

Results: As a result, the 2D gel showed totally 800 spots. HSP70 is one of down regulated proteins.

Conclusion: As conclusion, alteration of HSP70 expression was detected in Asterocytomas which has an important role in tumor survival and development, response to cell stress and induces apoptosis in cells. Three kinds of HSP70 are known in different cancers: HSP70-1, HSP70-2 and HSP70-8 which affect limitation of apoptosis system in cancer cells.

Keywords

HSP70 Heat-Shock Proteins Asterocytoma Proteomics Electrophoresis

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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