International Journal of Cancer Management

Published by: Kowsar

Comparing the Detection of Lymph Nodes Micrometastasis in Breast Cancer by the Hematoxylin and Eosin Staining Method (H&E) and the Immunohistochemical Method (IHC)

Shokouh Taghipour Zahir 1 , Shekofeh Shateri 2 , Mohammad Baghi Yazdi 3 , * , Pouria Barand 3 and Elham Zare 4
Authors Information
1 Full Professor of Clinical and Surgical Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran
2 General Practitioner, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran
3 Medical Student, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran
4 Resident of Internal Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran
Article information
  • International Journal of Cancer Management: November 2017, 10 (11); e11743
  • Published Online: November 30, 2017
  • Article Type: Research Article
  • Received: April 14, 2017
  • Revised: June 10, 2017
  • Accepted: November 1, 2017
  • DOI: 10.5812/ijcm.11743

To Cite: Taghipour Zahir S, Shateri S, Baghi Yazdi M, Barand P, Zare E. Comparing the Detection of Lymph Nodes Micrometastasis in Breast Cancer by the Hematoxylin and Eosin Staining Method (H&E) and the Immunohistochemical Method (IHC), Int J Cancer Manag. 2017 ; 10(11):e11743. doi: 10.5812/ijcm.11743.

Copyright © 2017, Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Ferlay J, Héry C, Autier P, Sankaranarayanan R. . Springer; 2010. Global burden of breast cancer. Breast cancer epidemiology; p. 1-19.
  • 2. Siegel R, DeSantis C, Virgo K, Stein K, Mariotto A, Smith T, et al. Cancer treatment and survivorship statistics, 2012. CA Cancer J Clin. 2012;62(4):220-41. doi: 10.3322/caac.21149. [PubMed: 22700443].
  • 3. Taghavi A, Fazeli Z, Vahedi M, Baghestani AR, Pourhoseingholi A, Barzegar F, et al. Increased trend of breast cancer mortality in Iran. Asian Pac J Cancer Prev. 2012;13(1):367-70. [PubMed: 22502702].
  • 4. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136(5):E359-86. doi: 10.1002/ijc.29210. [PubMed: 25220842].
  • 5. Stankov A, Bargallo-Rocha JE, Silvio AN, Ramirez MT, Stankova-Ninova K, Meneses-Garcia A. Prognostic factors and recurrence in breast cancer: experience at the national cancer institute of Mexico. ISRN Oncol. 2012;2012:825258. doi: 10.5402/2012/825258. [PubMed: 22830047].
  • 6. Sinha S, Nath J. Predictive and Prognostic Factors in Breast Cancer and their Association with ER PR HER2/neu Expression. J Carcinogenesis Mutagenesis. 2016;7(2). doi: 10.4172/2157-2518.1000263.
  • 7. Tjan-Heijnen VC, Buit P, de Widt-Evert LM, Ruers TJ, Beex LV. Micro-metastases in axillary lymph nodes: an increasing classification and treatment dilemma in breast cancer due to the introduction of the sentinel lymph node procedure. Breast Cancer Res Treat. 2001;70(2):81-8. [PubMed: 11768607].
  • 8. Garg M, Nagpal N, Sidhu DS, Singh A. Effect of Lump Size and Nodal Status on Prognosis in Invasive Breast Cancer: Experience from Rural India. J Clin Diagn Res. 2016;10(6):PC08-11. doi: 10.7860/JCDR/2016/20470.8039. [PubMed: 27504343].
  • 9. Weaver DL, Krag DN, Manna EA, Ashikaga T, Waters BL, Harlow SP, et al. Detection of occult sentinel lymph node micrometastases by immunohistochemistry in breast cancer. An NSABP protocol B-32 quality assurance study. Cancer. 2006;107(4):661-7. [PubMed: 17024757].
  • 10. Choudhury M, Agrawal S, Pujani M, Thomas S, Pujani M. Immunohistochemical detection of axillary lymph node micrometastases in node negative breast cancer patients using cytokeratin and epithelial membrane antigen. South Asian J Cancer. 2015;4(1):28-31. doi: 10.4103/2278-330X.149946. [PubMed: 25839017].
  • 11. Kohlberger P, Gantert M, Volk-Orlowska T, Kieback DG, Gitsch G. Immunohistochemical detection of lymph node metastases in node-negative breast cancer patients. Anticancer Res. 2001;21(1B):697-9. [PubMed: 11299828].
  • 12. Rutledge H, Davis J, Chiu R, Cibull M, Brill Y, McGrath P, et al. Sentinel node micrometastasis in breast carcinoma may not be an indication for complete axillary dissection. Mod Pathol. 2005;18(6):762-8. doi: 10.1038/modpathol.3800394. [PubMed: 15761490].
  • 13. Amir AS, Azar FP, Sahram N, Naser FM, Na'ma MR, Farid F, et al. Prevalence of occult metastases in axillary sentinel lymph nodes of breast carcinoma. Pol J Pathol. 2012;63(1):40-4. [PubMed: 22535605].
  • 14. Dabbs DJ, Fung M, Landsittel D, McManus K, Johnson R. Sentinel lymph node micrometastasis as a predictor of axillary tumor burden. Breast J. 2004;10(2):101-5. [PubMed: 15009035].
  • 15. Gobardhan PD, Elias SG, Madsen EV, van Wely B, van den Wildenberg F, Theunissen EB, et al. Prognostic value of lymph node micrometastases in breast cancer: a multicenter cohort study. Ann Surg Oncol. 2011;18(6):1657-64. doi: 10.1245/s10434-010-1451-z. [PubMed: 21153885].
  • 16. Viale G, Maiorano E, Mazzarol G, Zurrida S, Galimberti V, Luini A, et al. Histologic detection and clinical implications of micrometastases in axillary sentinel lymph nodes for patients with breast carcinoma. Cancer. 2001;92(6):1378-84. [PubMed: 11745213].
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